How to Control and to Maintain the Quality of Cell Cultures
Presenter: Oliver Wehmeier, acCELLerate GmbH
November 30, 2021
10:00-11:00 AM EST
Human or animal derived cell lines are major cornerstones for the development of alternative methods. An increasing number of animal tests can now be replaced by cell-based assay. The various validated methods provide a good predictivity and high precision, but the reliability of the assays very much depends on the quality of cell culture. Although considered as in vitro, cells are still living organisms.
There are many determinants which modulate the proper function of cells and have an impact on the outcome of an assay. To know what these determinants are and to understand how they can be controlled and standardized is the holy grail of cell culture. Ways to prevent microbial contamination, the importance of regular mycoplasma testing as well as the problem of cross contamination among cell lines will be discussed. Also, variances which are caused from the cells and the cultivation process itself will be addressed. The guidance for a Good Cell Culture Practice as the essential basis for running reliable and precise cell-based assays will be introduced.