Program

Monday, June 22, 2020
Interactive Training

9:00 am The Mechanics of Expert Literature Searching (3 hours) 

Rob Wright, MLS, Johns Hopkins University, Welch Medical Library

An expert literature search, which strives for comprehensiveness, requires a set of logically combined concepts and a thorough understanding of the controlled vocabulary, field searching, search operators, and other advanced searching capabilities of individual databases. This session will introduce a search-building workflow for PubMed within an animal replacement context. Specific steps in this workflow will be addressed, including using the MeSH Database to find preferred search terms, using field tags to format keywords, combining concepts and terms with Boolean operators, building searches using the PubMed advanced search feature, and using strategies to assess search quality.


Monday, June 22, 2020
Afternoon Session

12:45 pm Sign on
1:00 pm Welcome and Opening Statements
Kristie Sullivan, MPH, Physicians Committee for Responsible Medicine
Thomas Hartung, PhD, CAAT, Johns Hopkins University
1:15 pm

Keynote Address: Critical Thinking for Innovative Science
Warren Casey, PhD, National Institutes of Environmental Health Sciences 

1:45 pm

Science in the 21st Century: Is There Still a Place for Animal Experimentation?
Francesca Pistollato, PhD, European Commission Joint Research Centre
Kathrin Herrmann, PhD, CAAT, Johns Hopkins University

2:15 pm

Living the “Ph” in PhD: A Focus on Philosophy, History and Communication in 
Graduate Science Education

Gundula Bosch, PhD, MEd, R3 Center for Innovation in Science Education

2:45 pm Panel and Group Discussion
3:30 pm Innovative Science Happy Hour
4:45 pm Conclude Day One

 


Tuesday, June 23, 2020
Morning Session: Attend one track

Toxicology Track: In Vitro and In Silico Approaches 
Chair: Lena Smirnova, PhD, CAAT, Johns Hopkins University

9:00 am

Session Welcome
Lena Smirnova, PhD, CAAT, Johns Hopkins University

9:05 am

Non-animal Testing Methods for Acute Exposures to Ocular, Dermal, Mucosal and Respiratory Epithelia
Hans Raabe, MS, Institute for In Vitro Sciences

9:35 am

Use of Microphysiological Systems for Toxicology
Talita Marin, PhD, LNBio/CNPEM

10:05 am

Developmental Neurotoxicity and 3D Human Cell Models
Helena Hogberg, PhD, Johns Hopkins University

10:35 am

Break

10:45 am

A Large Multitask Neural Network for Chemical Hazards
Tom Luechtefeld, PhD, Insilica

11:15 am

Science Implementation: The Regulatory Context for New Approach Methodologies (NAMs) in Toxicology
Kristie Sullivan, MPH, Physicians Committee for Responsible Medicine

11:45 am

Q&A

12:00 pm

Break for Lunch

 

Biomedical Track: In Vitro and In Silico Approaches
Chair: Charu Chandrasekera, PhD, Canadian Centre for Alternatives to Animal Methods

9:00 am 

Session Welcome
Charu Chandrasekera, PhD, CCAAM 

9:05 am 

Virtual Patients: The Impending Revolution in Drug Development
Himanshu Kaul, D Phil, University of British Columbia 

9:35 am 

Xenofree Stem Cell Cultures to Study Diversity Factors of Disease
Ann Lam, PhD, Physicians Committee for Responsible Medicine 

10:05 am

Break

10:15 am 

Modeling Human Lung Diseases in Three-Dimension: Introducing Small Airway-on-a-Chip and Breathing-Smoking Lung-on-a-Chip Microfluidic Technologies
Kambez Benam, D Phil, University of Colorado, Anschutz Medical Campus

10:45 am 

3D Bioprinting as an In Vitro Platform for Complex Tissue Engineering 
Bhushan Mahadik, PhD, NIBIB/NIH Center for Engineering Complex Tissues, University of Maryland

11:15 am

Microphysiological Models: Challenges and Future Directions
Šeila Selimović, PhD, U.S. Department of Health and Human Services

11:45 am

Q&A

12:00 pm 

Break for Lunch


Tuesday, June 23, 2020
Afternoon Sessions

1:00 pm

Flash Poster Presentations I
20 presenters will have three minutes to present their research to attendees via Zoom, followed by facilitated virtual discussion in small groups

3:00 pm Virtual Meet the Mentors and Networking Session
4:30 pm Conclude Day Two

 


Wednesday, June 24, 2020
Morning Session: Attend one track

Toxicology Track: Integrated Approaches
Chair: Esther Haugabrooks, PhD, Physicians Committee for Responsible Medicine

9:00 am

Session Welcome
Esther Haugabrooks, PhD, Physicians Committee for Responsible Medicine

9:05 am

Overview of Evidence Integration and International Collaboration in Toxicology
Nicole Kleinstreuer, PhD, NICEATM, National Institutes of Health

9:35 am

A Case Study: Using New Approach Methods to Assess Estrogen Receptor Activity
Richard Judson, PhD, Environmental Protection Agency

10:05 am

Understanding Extrapolation Models: Can We Relate Our Health to Tests in Cells?
Andy Nong, PhD, Health Canada

10:35 am

Break

10:45am

Introduction to AOPs/MOA
Bette Meek, PhD, University of Ottawa

11:15 am

Q&A and Panel Discussion
11:30 am

AOP Interactive Segment: Find the Key Events
Facilitators:
Bette Meek, PhD, University of Ottawa
Noffisat Oki, PhD, AAAS STPF Fellow, National Institutes of Health

12:30 pm

Break for Lunch

 

Biomedical Track: Integrated Approaches
Chair: Kambez Benam, D Phil, University of Colorado, Anschutz Medical Campus

9:00 am

Session Welcome
Kambez Benam, D Phil, University of Colorado, Anschutz Medical Campus

9:05 am 

Disease-in-a-Dish: Moving Beyond Animal Models in Biomedical Research
Charu Chandrasekera, PhD, Canadian Centre for Alternatives to Animal Methods

9:35 am 

The Need to Prioritize ‘Replacement’ in Alzheimer’s Disease Research
Francesca Pistollato, PhD, European Commission Joint Research Centre

10:05 am 

Real-World Data Science to Untangle Environment and Genetics in Phenotype 
Chirag Patel, PhD, Harvard Medical School

10:35 am

Break

10:45 am 

3D Organotypic Tissue Cultures as Predictive Human Disease Models
Helena Hogberg, PhD, CAAT, Johns Hopkins University

11:15 am 

Public/Private Development Model for Mini-Brain Technology  
Lowry Curley, PhD, Axosim 

11:45 am

Q&A

12:00 pm 

Break for Lunch


Wednesday, June 24, 2020
Afternoon Sessions

1:00 pm

Flash Poster Presentations II
20 presenters will have three minutes to present their research to attendees via Zoom, followed by facilitated virtual discussion in small groups

3:00 pm

3:30 pm

4:00 pm

Virtual Laboratory Tours and Software Demonstrations I

Johns Hopkins University Statistical Methods and Applications for Research in Technology (SMART) Lab, Brian Caffo, PhD

Johns Hopkins University Data Science Lab, Brian Caffo, PhD

Institute for In Virto Science (IIVS), Hans Raabe, MS

4:30 pm Conclude Day Three

 


Thursday, June 25, 2020
Morning Session: Attend one track 

Toxicology Track:  Regulatory Application and Risk Assessment
Chairs: Maureen  Gwinn, PhD, Environmental Protection Agency &  Francesca Pistollato, PhD, European Commission Joint Research Centre

9:00 am

Session Welcome
Maureen Gwinn, PhD, DABT, Environmental Protection Agency
Francesca Pistollato, PhD, European Commission Joint Research Centre

9:05 am

Inhalation Risk Assessment Using Computational Fluid Dynamics and Three-Dimensional Respiratory Tissue
Douglas C. Wolf, DVM, PhD

9:35 am

Recent Advances in Animal Alternatives for Environmental Risk Assessment
Michelle Embry, PhD, Health and Environmental Sciences Institute

10:05 am

Break

10:15 am

Experimental Variability and Uncertainty In the Context of New Approach Methodologies for Potential Use in Chemical Safety Evaluation
Prachi Pradeep, PhD, US EPA

10:45 am

Regulatory Perspectives on Nonanimal Approaches for Chemical Assessment in Health Canada
Francina Webster, PhD, Health Canada 

11:15 am

Risk Assessment and Alternative Approaches: Considerations in Latin America
Mauricio Rodriguez, PhD, CropLife Latin America

11:45 am

Group Discussion and Q&A

12:30 pm

Break for Lunch

 

Biomedical Track: Clinical Relevance
Chair: Martin Stephens, PhD, CAAT, Johns Hopkins University

9:00 am 

Session Welcome
Martin Stephens, PhD, CAAT, Johns Hopkins University

9:05 am

Birth Tissue as a Plentiful Resource for Primary Human Cells for Use in Research and Development
Sharon Presnell, PhD, Amnion Foundation

9:35 am 

Solving the Human Translation Problem for Cardiovascular Drug Discovery 
Misti Ushio, PhD, TARA Biosystems

10:05 am 

Break

10:15 am 

Phase-0 Including Microdosing Approaches Using In-Vivo Human Data in the Preclinical Selection Process 
Tal Burt, MD, Phase-0/Microdosing Network

10:45 am 

Scientific Validity of Animal Free Antibodies for Use in Research and Risk Assessment
Rebecca Clewell, PhD, 21st Century Tox Consulting

11:15 am

The Urgent Need for Human-Specific Research: Widespread Failures of Animal Models Mean They’re Not an Option
Jarrod Bailey, PhD, Center for Contemporary Sciences

11:45 am 

Group Discussion and Q&A

12:30 pm

Break for Lunch  


Thursday, June 25, 2020
Afternoon Sessions

1:30 pm

Communicating Science to Journalists 
Denise-Marie Ordway, Harvard Kennedy School Shorenstein Center on Media, Politics and Public Policy

2:30 pm

Panel Discussion: Research Funding for Human Relevant Methods

  • Lingamanaidu (Ravi) Ravichandran, PhD, NIEHS, National Institutes of Health
  • Sue A. Leary, Alternatives Research and Development Foundation 
  • Lucie Low, PhD, NCATS, National Institutes of Health
3:15 pm Break

3:30 pm

4:00 pm

4:30 pm

Virtual Laboratory Tours and Software Demonstrations II

Center for Alternatives to Animal Testing (CAAT) In Vitro Lab, Helena Hogberg, PhD

US EPA Computational Toxicology Lab, Antony Williams, PhD

Sysrev Demo, TJ Bozada, BS

5:00 pm Conclude Day Four

 



Friday, June 26, 2020
Interactive Training

9:00 am Next Generation Risk Assessment (NGRA) case study: 0.1% Coumarin in a face cream (3 hours)
Maria Baltazar and Gavin Maxwell, Unilever

Several theoretical frameworks describing a tiered approach for NGRA have been published over the past few years (Berggren et al. 2017; Dent et al. 2018), but concrete next generation risk assessment (NGRA) examples of how to analyse, integrate and interpret all the data obtained from new approach methodologies (NAMs) in order to inform a safety decision are still lacking. Confidence in applying NAMs in a decision-making context will only come with learning by doing and sharing more case studies. Therefore, this session aims to share our recent NGRA case study work (Baltazar et al. 2020; Hatherell S. et al. 2020; Moxon et al. 2020) where we integrated currently available NAMs in a hypothetical safety assessment of 0.1% coumarin in a face cream to make a safety decision without using any animal data.

The objective of this session is to explore the different elements that form the basis of a NGRA approach, including an overview of the NAMs used (e.g. PBK modelling, Eurofins Safety44™ screen, BioMap® Diversity 8 Panel, high-throughput transcriptomics etc.,) followed by an in-depth discussion of the different elements of this case study: 1) exposure information and collation of existing information; 2) In vitro biological activity characterisation; 3) refinement steps; 4) margin of safety calculation and 5) risk assessment conclusion. Each section will include a short presentation followed by an interactive session (e.g. Q&A and/or polls). At the end of session participants will have the opportunity to share their confidence in the NGRA decision-making approach, any remaining uncertainties and suggest additional work to address identified data/knowledge gaps.

Baltazar MT, Cable S, Carmichael PL, Cubberley R, Cull TA, Delagrange M, Dent MP, Hatherell S, Houghton J, Kukic P et al. 2020. A next generation risk assessment case study for coumarin in cosmetic products. Toxicological Sciences (in press).

Berggren E, White A, Ouedraogo G, Paini A, Richarz A-N, Bois FY, Exner T, Leite S, Grunsven LAv, Worth A et al. 2017. Ab initio chemical safety assessment: A workflow based on exposure considerations and non-animal methods. Computational Toxicology. 4:31-44.

Dent M, Amaral RT, Da Silva PA, Ansell J, Boisleve F, Hatao M, Hirose A, Kasai Y, Kern P, Kreiling R et al. 2018. Principles underpinning the use of new methodologies in the risk assessment of cosmetic ingredients. Computational Toxicology. 7:20-26.

Hatherell S., Baltazar MT., Reynolds J., Carmichael PL., Dent M., Li H., Ryder S, White A., Walker P, AM. M. 2020. Identifying and characterizing stress pathways of concern for consumer safety in next generation risk assessment. Toxicological sciences (in press).

Moxon TE, Li H, Lee M-Y, Piechota P, Nicol B, Pickles J, Pendlington R, Sorrell I, Baltazar MT. 2020. Application of physiologically based kinetic (pbk) modelling in the next generation risk assessment of dermally applied consumer products. Toxicology in Vitro. 63:104746.

12:00 pm Awards and Recognition Ceremony
1:00 pm Conclusion of the Summer School