Register now for the next ASCCT webinar:
Thursday, Jun 8, 2017
11:00 AM - 12:00 PM ET
Title: Combination of multiple neural crest migration assays to identify environmental toxicants from a proof‑of‑concept chemical library
Presenter: Johanna Nyffeler, In Vitro Toxicology and Biomedicine, University of Konstanz, Germany
Many in vitro tests have been developed to screen for potential neurotoxicity. However, only few cell function-based tests have been used for comparative screening, and thus experience is scarce on how to confirm and evaluate screening hits. We addressed these questions for the neural crest cell migration test (cMINC). After an initial screen, a hit follow-up strategy was devised. A library of 75 compounds plus internal controls (NTP80-list), assembled by the National Toxicology Program of the USA (NTP) was used. It contained some known classes of (developmental) neurotoxic compounds. The primary screen yielded 23 confirmed hits, which comprised ten flame retardants, seven pesticides and six drug-like compounds. Comparison of concentration–response curves for migration and viability showed that all hits were specific. The extent to which migration was inhibited was 25–90%, and two organochlorine pesticides (DDT, heptachlor) were most efficient. In the second part of this study, (1) the cMINC assay was repeated under conditions that prevent proliferation; (2) a transwell migration assay was used as a different type of migration assay; (3) cells were traced to assess cell speed. Some toxicants had largely varying effects between assays, but each hit was confirmed in at least one additional test. This comparative study allows an estimate on how confidently the primary hits from a cell function-based screen can be considered as toxicants disturbing a key neurodevelopmental process. Testing of the NTP80-list in more assays will be highly interesting to assemble a test battery and to build prediction models for developmental toxicity.
Registration is free and open to anyone. Please feel free to share with your colleagues.
September 21-22, 2017
FDA CFSAN Building
5001 Campus Drive
College Park, Md
Plenary session, Day 1
Topic: Alternatives to acute systemic toxicity
Speakers: Anna Lowit, US EPA OPP & Dan Wilson, Dow Chemical Company
Plenary session, Day 2
Topic: Lautenberg Chemical Safety Act (LCSA) implementation
Speaker: Louis “Gino” Scarano, US EPA OPPT
Save the date! The next ASCCT meeting will offer presentations on scientific and policy advancements related to acute toxicology and TSCA reform, as well as free communications. Most talks will be selected from submitted abstracts.
Visit the meeting home page for more information.
- Single-cell analysis reveals that silver nanoparticle exposure leads to multi-nucleation through defective cell division | Ellen Garcia, Virginia Tech
- Modeling a complex in vivo response in vitro: Exploring heterogeneity and mechanisms associated with ozone adaptation | Emma Bowers, University of North Carolina
- Rapid 3D Bioprinting: an Enabling Technology for Creating Functional Tissue Models | Shaochen Chen, University of California, San Diego
- Human Primary In Vitro Systems for Translational Drug Safety and Mechanisms of Toxicity | Ellen Berg, Bioseek/DiscoverX
- Integrating in silico predictions with PBPK modeling | Michael Lawless, SimulationsPlus, Inc.
- The GARD assay for skin and respiratory sensitization | Andy Forreryd, Lund University
- COSMOS: Automated in silico tools for in vitro to in vivo extrapolation| Alicia Paini, European Commission Joint Research Centre
- Threshold of Toxicological Concern â€“ an approach for safety assessment and its applicability to cosmetics-related chemicals | Chihae Yang, Altamira, LLC and Molecular Networks
- Medical Device Irritation Testing: An In Vitro Alternative | Kelly Coleman, Medtronic Biomaterials Dept
- Developing Confidence in 21st-Century Risk Assessments | Craig Rowlands, Dow Chemical Company
- COSMOS DB: A New Database of Toxicological Information to Support Knowledge Discovery | Mark Cronin, Liverpool John Moores University and COSMOS project coordinator
- High Content Imaging Approaches in Neurotoxicology and Neurodegeneration Research | Marcel Leist, University of Konstanz and CAAT Europe
- EURL ECVAM's Approach to Validation of Alternative Methods | Valerie Zhuang, EURL ECVAM
- Using the AOP Framework to Develop HTS Assays for Thyroid-Disrupting Chemicals | Katie Paul, US EPA
- The Role of Non-Animal Methods in the Regulation of Nanomaterials in the United States | Erik Janus, Steptoe and Johnson LLP (Recording not available)
- Human Induced Pluripotent Stem Cell-derived Model Systems | Maureen Bunger, Cellular Dynamics
- Incorporating New Technologies into Toxicity Testing and Risk Assessment | Russell Thomas, The Hamner Institutes for Health Science
- The Virtual Embryo | Nicole Kleinstreuer, US EPA
- Tox21 Program Update | Raymond Tice, National Toxicology Program, NIH
- Evidence-Based Toxicology (EBT) for the 21st Century | Martin Stephens, Johns Hopkins University
- Effectopedia: The Online Encyclopedia of Adverse Effect Pathways | Gilman Veith and Hristo Aladjov, International QSAR Foundation
- MetaPath: A Metabolism Pathway Database | Patricia Schmeider, US EPA
Fifth Annual Meeting of the American Society for Cellular and Computational Toxicology
Thank you to all who helped make our fifth meeting the best yet. Attendees were treated to plenary lectures on read across by Dr. Thomas Hartung of Johns Hopkins University and on stem cells in toxicology by Dr. Mahendra Rao of Mahendra Rao, LLC. Sixteen presentations showcasing advances in the field of toxicology, as well as a mentoring session with young scientists, a poster session, and dynamic panel discussion rounded out the program.
We were honored to host the wife and brother of the late Dr. Edward Carney to award the 2nd annual Ed Carney Award for Predictive Toxicology, which went to Emma Bowers for her presentation Modeling a complex in vivo response in vitro: Exploring heterogeneity and mechanisms associated with ozone adaptation. After winning the William and Eleanor Cave Award for his career achievements in in vitro toxicology, Dr. Ray Tice established the Tox21 Student Award, which will be given for the next five years. This year, the award went to Ellen Garcia for her presentation Single-cell analysis reveals that silver nanoparticle exposure leads to multi-nucleation through defective cell division.
Finally, members elected Ellen Berg to the Board of Directors
ASCCT members can view all presentations.
Fifth Annual Meeting of the American Society for Cellular and Computational Toxicology
Fourth Annual Scientific Meeting of the American Society for Cellular and Computational Toxicology: Promises and Challenges of a More Flexible Approach to Toxicology Testing
The fourth annual ASCCT meeting explored new approaches to toxicology testing with plenary lectures given by Warren Casey, Director of NICEATM and Craig Rowlands from the Dow Chemical Company. The meeting featured a diverse poster session, a panel discussion on the assessment and application of IATA, and ten oral presentations chosen from a very competitive field of abstracts. Members elected two new members to the Board of Directors: Gertrude-Emilia Costin and Shaun McCullough. View Program. ASCCT members, click here to view all presentations.
Third Annual Scientific Meeting of the American Society for Cellular and Computational Toxicology: Where Chemistry and Biology Meet: AOPs as a Framework for Advancing Toxicology
Dr. Robert Kavlock of the US Environmental Protection Agency opened the meeting with a discussion on the development of the Adverse Outcome Pathways concept, in which AOPs offer a biological context to facilitate the development of Integrated Approaches to Testing and Assessment (IATA) for regulatory decision-making. Drs. Jennie Larkin and Ajay Pillai from NIH discussed BD2K and LINCS, two community-building programs. Other presenters explored the development of different tools used to test toxicity. A poster session and reception concluded the meeting. View Program. ASCCT members, click here to view all presentations.
Second Annual Scientific Meeting of the American Society for Cellular and Computational Toxicology: The Future Is Here: Practical Applications of Emerging Scientific Tools
Human Organs on Chips as Replacements for Animal Testing, the keynote address given by Donald E. Ingber, M.D., Ph.D., of the Wyss Institute at Harvard, kicked off the meeting which featured a dozen lectures and poster presentations on making toxicology a more human-relevant science. Other topics included methods to replace live animals in eye irritation tests and computer-based virtual embryos that show how chemical exposures might affect developing embryos. View Program.
First Annual Scientific and Business Meeting of the ASCCT
The first annual ASCCT meeting focused on advances in the fields of in vitro and computational toxicology. Dr. Melvin Andersen, associate director of The Institute for Chemical Safety Sciences at The Hamner Institutes for Health Sciences and ASCCT board member, gave a plenary lecture on Computational Cellular Pathway Modeling: Combining Key In Vitro and In Silico Tools to Enhance Modern Safety Assessment. Dr. Suzanne Fitzpatrick, senior advisor for toxicology at the U.S. Food and Drug Administration gave a plenary lecture on an Overview of the FDA-DARPA-NIH Collaboration on Human/Organ on a Chip. View Program.
Institute for In Vitro Sciences Practical Methods for In Vitro Toxicology Workshop
Attendees gained hands-on laboratory experience and instruction in the practical application of the lectured topics. Topics for the three and a half day course included: (i) international regulatory acceptance status of in vitro assays, (ii) predicting organ specific toxicity (e.g., ocular, dermal and hepatic), (iii) using cell and organ culture models with histological, biochemical, and molecular endpointsm, and (iv) data interpretation.
2010 In Vitro Alternatives Forum
Thank you for contributing to a successful forum. Read the post-meeting report.
8th World Congress for Alternatives and Animal Use
Thank you for helping to make the ASCCT/ESTIV luncheon session a success. A manuscript outlining the discussions that took place during the session has been published. You can view the entire proceedings here.